02-P005 A mechanism for “brain sparing” during dietary restriction in Drosophila

phology. We have generated a Tg(phd3:GFP)/+; vhl1/+ transgenic line, which exhibit robust GFP expression in the vhl1/vhl1 background, essentially acting as an in vivo fluorescent reporter to identify the mutant cells/embryos. To induce VHL kidney tumors in fish, we have utilized this transgenic line to generate donor embryos and transplanted the mutant blastomeres to the wild type host embryos, specifically targeting these mutant cells to the renal primordium. We report that Tg(phd3:GFP) line is a live reporter for monitoring hypoxia and the induction of HIF signaling. We shall present our observations on the fate of the vhl1 mutant cells in the chimeric fish and also our analysis for the possible occurrence of neoplasia in these chimeric fish.